3^rd International Conference on
Biomedical Engineering Instrumentation

In this talk, I will present how to gaze at the health status of humanity and the Earth in a grain of sand and find solutions for preventive precision medicine against a global pandemic such as COVID-19. Quantum plasmonic-based speedy analytical nano-optofluidic diagnostic system (SANDs) can help us to predict and stop the spread of infectious diseases. As an example of intelligent SANDs, integrated molecular diagnostic systems that comprise three key elements are developed: (1) self-contained sample preparation and liquid biopsy on-chip, which allows a rapid sample-to-answer readout platform; (2) ultrafast quantum plasmonic amplification of DNA, RNA, and protein biomarkers; (3) interface of smartphone optical system.
I will also describe exosome detection via the ultrafast-isolation system (EXODUS). The EXODUS via nanotechnology with resonator allows automated label-free purification of exosomes from varied biofluids. Two coupled oscillators of EXODUS generate dual-frequency transverse waves on nanoporous membranes to eliminate the fouling layers through acoustofluidic streaming and obtain a high speed, purity, and yield. As an example of EXODUS applications, we demonstrated the significantly improved purification method of exosomes from cancer patients’ urine samples. We validated the practical relevance in exosomal RNA profiling to compare enriched pathways of kidney and bladder cancer.
For personalized precision nanomedicine, I will present the development of brain organoid microphysiological analysis platforms (MAP) on-chip with nano-biological quantum sensors and therapeutic modulations, which will benefit from predicting the most effective treatment of diseases for each patient. Human-induced pluripotent stem cells-based brain organoid MAP provides an ideal model to address fundamental questions of neuropathogenesis and find solutions for neurodegenerations. In addition, patient-derived organoids can recapitulate patient responses and help personalized medicine. Smart SANDs, EXODUS, and organoid MAPs by the convergence of biology, chemistry, physics, and engineering will impact quantitative life sciences and precision nanomedicine.

Prof. Luke P. Lee received his BA in Biophysics and Ph.D. in Applied Physics and Bioengineering from UC Berkeley. He joined the faculty at UC Berkeley in 1999 after more than a decade of industry experience. He became the Lester John and Lynne Dewar Lloyd Distinguished Professor and Arnold and Barbara Silverman Distinguished Professor at Berkeley. He also served as the Chair Professor in Systems Nanobiology at the ETH Zürich from 2006 to 2007. He founded the Biomedical Institute for Global Healthcare Research & Technology (BIGHEART). He served as Associate President (International Research and Innovation) and Tan Chin Tuan Centennial Professor at the National University of Singapore from 2016 to 2018. He is the founding director of the Institute for Quantum Biophysics, Sungkyunkwan University, Korea. He is a Fellow of the Royal Society of Chemistry and the American Institute of Medical and Biological Engineering. His work at the interface of biological, physical, and engineering sciences for medicine has been recognized by many honors, including the IEEE William J. Morlock Award, NSF Career Award, Fulbright Scholar Award, and the HoAm Prize. Lee has over 350 peer-reviewed publications and over 60 international patents filed. His current research interests are quantum biological electron transfer (QBET) in living organisms, early detection of infectious diseases, and neurodegenerative diseases.